pAAV, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus), plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein pBR322, beta-lactamase maintenance, CMV promoter, expresses ABE8.13-d in mammalian cells CMV Working in human cells, Liu and coworkers used adenine base editing to correct a point mutation that causes the iron-storage disorder hemochromatosis and to install mutations that protect against sickle cell anemia. pcDNA3.1(+), Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus pEGFP-N1 p2T-CMV-BlastR, Express YE1-BE3-FNLS pcDNA3.1(+), Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus pBR322 CMV, Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter COVID-19 and Coronavirus Plasmids & Resources page, Genome pBR322, beta-lactamase maintenance, CMV promoter, expresses ABE8.17-d in mammalian cells px601, v5 AAV-CBE C-terminal; U6-protospacer (Synthetic), AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA pCambia, CRISPR/Cas9-mediated base editing in dicots. p415, Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells pCMV, Expresses SaBE3-Gam in mammalian cells Adenine base editing “is a really exciting addition to the genome-engineering toolbox,” comments Feng Zhang of the Broad Institute of MIT and Harvard, whose group pioneered the use of CRISPR for mammalian genome editing. APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3, Expresses hA3D-BE3 in mammalian cells What is an MTA/Who is authorized to sign? p2T-CMV-BlastR, eA3A-BE4 T31A-nSpCas9-UGI-UGI (Synthetic), C-to-T/G/A base editor CMV, CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP p415, Expressing base editor eA3A-BE3 in yeast cells CMV pCMV_ABEmax_P2A_GFP, human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP (Synthetic), CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP Adapted from lentiCRISPRv1, Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells CMV pZD_OsU3gYSA_HolgerCas9_NPTII, Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pBT195, ABEmax(TadA E59A, TadA* V106W) (Homo sapiens), Expresses ABEmaxAW in mammalian cells pCAG-CFP, human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic), CAG promoter expression plasmid for human codon optimized LbBE1.4 We use the information you provide to make your reading experience better, and we will never sell your data to third party members. CRISPR is ideal for inserting and deleting DNA sequences at targeted locations in a genome, but base editing has the edge for single-base changes. pX330 pCAG-CFP, DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic), CAG promoter expression plasmid for human codon optimized enAsBE1.1 pcDNA3.1(+), Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 ) (optional). CMV Aicda Aid, Arp2, Expresses mA1-BE3 in mammalian cells CMV, eUNG-BE4max(R33A)-ΔUGI (Rattus norvegicus), CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP pBT195, Expresses evoAPOBEC1-BE4max-NG in mammalian cells APOBEC3A A3A, ARP3, PHRBN, bK150C2.1, human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic), CAG promoter expression plasmid for human codon optimized AsBE1.1 p2T-CMV-BlastR, eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI (Synthetic), C-to-T base editor, NG PAM pBT195, express evoCDA1-BE4max in mammalian cells p415, rAPOBEC1-XTEN-zCas9-UGI-NLS (Rattus norvegicus), expression of an optimized Cas9 for base-editing in zebrafish CMV, ABEmax(TadA E59A TadA* D108Q) (Homo sapiens), pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells We use cookies to help provide and enhance our service and tailor content and ads. pcDNA3.1(+), nSpyCas9-rAPOBEC3-ID-1058 (Rattus norvegicus), Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor. Adapted from pLentiCRISPRv1, Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized) Chemical & Engineering News will not share your email address with any other person or company. “There is also the potential to develop these enzymes as therapeutics for genetic diseases caused by specific mutations. pCMV, Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2, empty sgRNA cloning vector with MS2-AID3f 664 patients and 154 CFTR mutations represented in an organoid biobank, Adenine base editors enable efficient repair of nonsense mutations in CFTR, xCas9 increases the target scope of CFTR repair in our biobank, Adenine base editors cause no detectable off-target effects during repair. pZD_OsU3gYSA_HolgerCas9_NPTII, CRISPR/Cas9-mediated base editing in Arabidopsis. APOBEC3A A3A, ARP3, PHRBN, bK150C2.1, Expresses hA3B-BE3 in mammalian cells MSCV Retroviral backbone. The new method also makes many orders of magnitude fewer indels. pZDgRNA_Cas9ver.2 (D10A)_HPT, Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pICH47742, STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing By continuing you agree to the use of cookies. CMV 96930), expresses ABE8.8-m in mammalian cells There is a problem with the plasmid I received. Further advancements have been made by optimizing expression of the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM). What do I need to know about the customs and importation process for my country? pLL3.3, Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) pCMV, Mammalian expression vector for xCas9(3.6)-ABE Chemistry matters. pEF, Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter. pCMV, Expresses eBE-S3 in mammalian cells How can I be notified when a plasmid from a specific lab or paper is available? pJY-RpABE, Targeted A to G in rice pEF, Mammalian expression plasmid for BE4-PpABOBEC1H122A However, beyond relatively easily targeted organs, genomic therapies like this will require significant advances in methods to deliver the large biological molecules involved to the diseased tissues.”. CAG, NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP (Other), CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. Have questions about your order, deposit, or a plasmid? Adapted from lentiCRISPRv1, AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA Here, we describe a cystic fibrosis (CF) intestinal organoid biobank, representing 664 patients, of which ~20% can theoretically be repaired by ABE. pCMV, Mammalian expression vector for xCas9(3.6)-BE4 pCMV, Lentiviral vector for constitutive expression of BE3 (not codon optimized)