Observe all federal, state and local regulations. Long Term Exposure: No information available, Injection: Corrosive to mucus membranes. C. Relevant Identified Uses of the Substance or Mixture: Observe all Federal, State and Local Regulations. Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Employees should wear appropriate protective clothing and equipment to prevent skin contact with this material. b. sheets, Supplier generation sequencing, Genes & Individual lots are screened for endotoxins using a Limulis Abocyte Lysate (LAL) assay. DOCUMENT NUMBER: IDT-086 Platform, Product Absorb with appropriate sorbent material and place spillage in appropriate container for disposal according to local regulations. Nuclease-free reagents for resuspending, diluting, and storing oligos. It is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.Composition:10 mM Tris-HCl (pH 8.0)0.1 … reports, DNA Oligo Carcinogen Status: reports, DNA Oligo Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners, and may be registered in the USA and/or other jurisdictions. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. Product Identifiers Product Name: IDTE Synonyms: N/A Index #: N/A CAS #: N/A B. tutorials, Technical Employee must wear appropriate protective gloves to prevent contact with this material. Long Term Exposure: No information available, Eye Contact: Gloves: Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search. IF IN EYES: Rinse cautiously with water for several minutes. protocols, Safety data integrations, User guides & user and need to request a new account, © 2020 Adaptive Biotechnologies. LOG Bioconcentration Factor (BCF): Wash exposed area with soap and water. Signal Word: Danger Reports, How clonoSEQ Inquiry, Importance of Use prudent laboratory practices for handling and storage of chemicals. Remove contaminated clothing. Note to Physician: Index #: N/A させ、Tm値を上昇させる方法, b) ターゲット遺伝子の異なる箇所で、プライマー・プローブを設計する方法, a)LNAという修飾塩基を用いる方法, 溶解前に遠心を行い、DNAペレットをチューブの底に落として下さい。, 最終濃度が100ng/µLとなるように、TE buffer (10 mM Tris, 0.1 mM EDTA, pH 7.5～8.0)もしくは、nuclease free waterを加えて下さい。, 30分間の室温静置後、20秒間のボルテックスを行なって下さい。, 10,000gで1分間遠心後、-20℃で分注及び保管を行なって下さい。, 999µL≒1mLのnuclease free waterを入れたチューブへ、100ng/µLのストックから1µLを移し、100pg/µLのDNA希釈溶液を作ります。, 1mLのDNA希釈溶液のうち1～2µLを大腸菌への形質転換に用います。. Clinicians, Clonoseq No information available. A standard scale IDT PrimeTime® qPCR Assay containing oligonucleotide primers and probes was hydrated in IDTE Buffer to 40X. Clothing: Remove, Skin Contact: Oligo stability in 3 storage mediums at –20°C, 4°C, and 37°C.Stability of oligos stored in TE buffer (IDTE, pH 8.0), nuclease-free water, or dry, for up to 24 months was measured in a qPCR assay by assessing ∆C q of the assay. Each lot is tested using our RNaseAlert® and DNaseAlert™ reagents to document the absence of any detectable nuclease activity. Synonyms: N/A sheets, Supplier This 1X TE Buffer is a component of the PureLink 96 Plasmid Purification System, now offered separately. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg . I am a Resuspend assay in IDTE buffer (Cat no. 1. Integrated DNA Technologies Short Term Exposure: Corrosive to mucus membranes. Precautionary Statements: MRD, Technical Acute Health Hazard (Edetic acid), Skin Contact: Entry, PrimerQuest A. GHS Label Elements, Including Precautionary Statements, I. Pictogram: NOT APPLICABLEII. No. First use Nuclease Decontamination Solution to eliminate nucleases from your work space, and then resuspend, anneal, or dilute, and store your oligos with our nuclease-free reagents. Long Term Exposure: No information available, Inhalation: Target Effects: Works, Product We do not recommend long-term storage of oligos in water, as this can increase the risk of oligo degradation. Remove from exposure area to fresh air immediately. Call a physician.