What are the main concerns with the technology and its application to human biology? CRISPR Why is this technology so exciting and with such great potential? (���ؙ�>]�E��e�ˆZ�3��c�L�e`�;�3�"h��53��ѿ�e%|� &|4E��p�>b�L��\y�p���X8R�X������Ċ?g�����"����0�����1���( �x��v,r��,P*b��P�s�Wsw:��V���W�Ɔ�>���Y��0 /�t�-����2NJ���iN�[gC����w�A���}�f��rx��m�R�n�>�5�'�%E�օ�柶f�eIE���lY��fIZ�DJ�bJ)LJ�ֈ�_K��G6�&{7��#�Eg$7}Ҟ�i�:�`��{e]++���~v2������'G ~�P�> ���\FqT�8P�(�&�:��Qe5P����e*$� �D�$3+�A��@�n�EV�0.H.a8�!��Qt�V��nEw�L.�,��GR (�~�t_�}����A7�� Ny/y���e�S)0m�5���k?��^���[�P�o�֏s��~?d�.u+d����lϓ���>}�f�ze�_`�k���g�]����Q�S���_�U���U瘌��������""\`�2& target both a and b (long and short genes)? Does your target sequence have GGN19GG or CCN19CC sequence? h�b```����}� CRISPR stands for “clustered regularly interspaced short palindromic repeats”, but this has little to with how the technology is … h޴Vmk�0�+��1�u�-YPyiha�J����n��I\w����d+��ԥt+�:I�"?��. CRISPR: hot, hot, hot 166 CRISPR is the latest technique for genome engineering and is generating tons of excitement due to its versatility, high specificity, and ease of use. %PDF-1.6 %���� h�bbd```b``9 "��ɼD2M��+�"�@���Ď�b�9b��SU@l�@�Cz�-� $�_��C��M �eU�����; D.��E q�`���H��`�T�HS��\i,~�k6ȅ��,��>z�� t��/ �\� endstream endobj startxref 0 %%EOF 131 0 obj <>stream Due to its ability to perform site directed DNA cleavage and to trigger CRISPR (clustered regularly interspaced short palindromic repeats) is an immunological defense mechanism employed by bacteria against invading pathogens. �/E:�B�㵞?�4��S9��F�Ġ���ZC/"i�p�N�a���J��� �K�紿�$��$��!��O&�q�4�ha�H�|�B�T�y j��k34R��+���ic:�0�Ef�+��z������e'�q[H:�~\� ��F\�g���(,��=R-" �D����+88��~݆_t�6����):nZ��mZ��V�iA� �ls4n�W��S�:�1m~�3�D����4�����L7�������Srߴ���H��iP��u����ktm'\�#� Kh�6 endstream endobj 87 0 obj <>stream ��0� ��LP���ւ)�8��N�P9�(�dL;�A,ч�]�J���6L�"E΄���������®b��w��bo�� �o�2ۮP��G��o����M���m���\ӽo�o��iS)�Jf���l��"�o"��"_Ll9#���>���&]�a5]V�`~�x��. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease. ce`a�� a���``8 ���P���à����������8�A5�a5�}ƛ�Y000800�``f���}�=8q�(�w%/Ԋ�z~�h�v�c���z��)Q�N����t}���MFb��$|7� sU�(�Kx� target within an exon? EǑ��+��G�&�SK](���7�"����r��҃gJ"�M�T5H=��(��[��ٌc̱H��[0�V��#�fSB�ٳ�g��V��S��>�mu����M[p"L)Gp6�����r���D)��(Җ���C�Ş��=e�������Ӥ �uR�P�X%�f endstream endobj 88 0 obj <>stream �(`��@,�$p��E�e`��'+�A�,�zy9�b!� x�Լy���?^U}��=���3;;��,,���궜r߰��.r�!r# ��D�h"ނ��\P#1�ă@�Q1��q��!�;�{�f������������~���~��s�S�d��Ȋ���i�.��!�B�1mْ����@�"�����y�+��EH������j���'7~����f]?k���? 83 0 obj <> endobj 117 0 obj <>/Filter/FlateDecode/ID[<6C4A188080A9723B09E94E3552486C6D>]/Index[83 49]/Info 82 0 R/Length 161/Prev 1485234/Root 84 0 R/Size 132/Type/XRef/W[1 3 1]>>stream